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STEMCELL Technologies Inc bit 9500 serum substitute
Bit 9500 Serum Substitute, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bit-9500/bit+9500/bio_rxiv__2025__07__15__664865-166-25-33
Average 90 stars, based on 1 article reviews
bit 9500 serum substitute - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Reprogramming T cells and hematopoietic cells
Article Snippet: The colonies were harvested using TrypLE (Invitrogen) and placed in serum-free embryoid body (EB) basal media [containing IMDM, NEAA, Glutamine (Invitrogen) and 20% BIT-9500 (Stem Cell Technologies) and ROCK inhibitor H1152 in low-attachment plates to facilitate aggregate formation.

Article Title: Monocytic Subclones Confer Resistance to Venetoclax-Based Therapy in Acute Myeloid Leukemia Patients
Article Snippet: SFM is composed of IMDM (Gibco), 20% BIT-9500 (Stemcell Technologies), 10ug/ml LDL (Low Density Lipoprotein, Millipore), 55uM 2-Mercaptoethanol (GIBCO) and 1% Pen/Strep (Gibco).

Article Title: SMYD2 lysine methyltransferase regulates leukemia cell growth and regeneration after genotoxic stress
Article Snippet: AML-193, a cytokine-dependent human leukemia cell line was purchased from ATCC (CRL958) and cultured in IMDM supplemented with BIT-9500 (5%, Stem Cell Technologies, Canada), FBS (5%), GM-CSF (5ng/ml), L-Glu (1%) and P/S (1%).

Article Title: Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling.
Article Snippet: HNSPC proliferation media is composed of DMEM/F-12 50/50 with L-glutamine and 15mM HEPES (Corning), 20% (v/v) BIT-9500 (STEMCELL Technologies), 1% (v/v) antibiotic/antimycotic (penicillin/ streptomycin/amphotericin; Thermo Fisher Scientific), supplemented with growth factors EGF (40 ng/mL; PeproTech), bFGF (40 ng/mL; PeproTech), and PDGF-AB (40 ng/mL; PeproTech).

Article Title: Biophysical Characteristics Reveal Neural Stem Cell Differentiation Potential
Article Snippet: Briefly, undifferentiated cells were grown as adherent cultures on fibronectin coated flasks in basal medium [DMEM/F12 (Invitrogen, Carlsbad, CA), 20% (v/v) BIT-9500 (Stem Cell Technologies, Vancouver BC), 1% (v/v) antibiotic/antimycotic (Invitrogen, Carlsbad, CA)] supplemented with the following mitogenic growth factors: 40 ng/ml EGF (BD Biosciences, Bedford, MA), 40 ng/ml FGF (BD Biosciences, Bedford, MA), and 40 ng/ml PDGF (Peprotech, Rocky Hill, NJ).

Article Title: Myosin-II mediated traction forces evoke localized Piezo1-dependent Ca 2+ flickers
Article Snippet: Briefly, undifferentiated cells were grown as adherent cultures on fibronectin (Fisher Scientific)-coated flasks in basal medium containing DMEM/F12 (GIBCO), 20% BIT-9500 (Stem Cell Technologies), and 1% antibiotic/antimycotic (Invitrogen) supplemented with the following growth factors: 40 ng/ml epidermal growth factor (EGF) (BD Biosciences), 40 ng/ml fibroblast growth factor (FGF) (BD Biosciences), and 40 ng/ml PDGF (Peprotech). hNSPCs were passaged approximately every 5–7 days using cell dissociation buffer (Invitrogen) and split 1:2.

Article Title: Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling
Article Snippet: HNSPC proliferation media is composed of DMEM/F-12 50/50 with L-glutamine and 15 mM HEPES (Corning), 20% (v/v) BIT-9500 (STEMCELL Technologies), 1% (v/v) antibiotic/antimycotic (penicillin/streptomycin/amphotericin; Thermo Fisher Scientific), supplemented with growth factors EGF (40 ng/mL; PeproTech), bFGF (40 ng/mL; PeproTech), and PDGF-AB (40 ng/mL; PeproTech).




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